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TM3'seq

Tagmentation-Mediated 3'-enriched RNA-seq

| Home | Analysis Pipeline | mRNA Extraction Protocol | TM3’seq Protocol | Liquid-Handling Robot Protocols |

Liquid-handling robot protocols

The mRNA extraction and TM3’seq protocols were optimized to work on 96-well plates, and therefore they can be used as reference to develop protocols for liquid-handling platforms.

We have implemented both protocols in CyBio® FeliX pipetting robot (Analitik Jena). The main protocols, subroutines, and set-up files for this specific liquid-handling platform are available under request. Please contact the authors:

Luisa F. Pallares - pallares@princeton.edu

Julien F. Ayroles - jayroles@princeton.edu